pftA

168

pyruvate transporter

Locus: BSU_28910

Molecular weight: 15.57 kDa

Isoelectric point: 9.82

Protein length: 146 aa

Gene length: 441 bp

Function: uptake of pyruvate

Product: pyruvate transporter

Essential: no

Synonyms: pftA, ysbA, lrgA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1380 (Galperin et al., 2021)

Gene

Coordinates: 2,955,209  2,955,649

Phenotypes of a mutant

• strongly impaired growth with pyruvate as the single carbon source PubMed
• ''pftA-pftB'' mutant is delayed in pellicle formation PubMed
• a ''ywbH-ywbG yxaK-yxaC pftA-pftB'' triple mutant is severely delayed in pellicle formation PubMed

The protein

Catalyzed reaction/ biological activity

• uptake of pyruvate PubMed

Protein family

• CidA/LrgA family (with YwbH, according to UniProt)

Structure

• P94515 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: pftA-pftB

  Description: PubMed

  Regulation

  • induced by acetate PubMed
  • induced by pyruvate (LytT) PubMed
  • subject to carbon catabolite repression (CcpA) PubMed

  Regulatory mechanism

  • LytT: activation, PubMed, in lytT regulon
  • CcpA: repression, (PubMed, in ccpA regulon
  • Kre: repression, in kre regulon

  Additional information

  • the pftA-pftB operon is strongly upregulated in a ''kre'' mutant PubMed
  • the pftA-pftB operon is strongly upregulated in a ''cshA'' mutant PubMed

Biological materials

Mutant

• MGNA-B013 (ysbA::erm), available at the NBRP B. subtilis, Japan
• GP1554 (''pftA-pftB::spc''), available in Jörg Stülke's lab
• BKE28910 (pftA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCTTCACCTCTTTCT,  downstream forward: _UP4_TAAGGGAAACAGGAGGTTCT
• BKK28910 (pftA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCTTCACCTCTTTCT,  downstream forward: _UP4_TAAGGGAAACAGGAGGTTCT

References

Reviews

Loading

Original Publications

Loading