Difference between revisions of "TsaC"

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[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 09:35, 1 June 2015

  • Description: L-threonylcarbamoyl-AMP synthase, biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A) (together with TsaB-TsaD-TsaE)

Gene name tsaC
Synonyms ipc-29d, ywlC
Essential no
Product L-threonylcarbamoyl-AMP synthase
Function tRNA modification
Gene expression levels in SubtiExpress: tsaC
MW, pI 36 kDa, 5.82
Gene length, protein length 1038 bp, 346 aa
Immediate neighbours ywlD, ywlB
Sequences Protein DNA DNA_with_flanks
Genetic context
YwlC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YwlC expression.png















Categories containing this gene/protein

translation

This gene is a member of the following regulons

DnaA regulon

The gene

Basic information

  • Locus tag: BSU36950

Phenotypes of a mutant

Database entries

  • DBTBS entry: no entry
  • SubtiList entry: [1]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
    • conversion of L-threonine, bicarbonate/CO2 and ATP to give the intermediate L-threonylcarbamoyl-AMP (TC-AMP) and pyrophosphate as products PubMed
    • biosynthesis of the hypermodified base threonylcarbamoyladenosine (t(6)A), a universal modification found at position 37 of ANN decoding tRNAs, which imparts a unique structure to the anticodon loop enhancing its binding to ribosomes in vitro (shown for the corresponding E. coli protein YrdC) PubMed
  • Protein family: SUA5 family (according to Swiss-Prot)
  • Paralogous protein(s):

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1HRU (the homolog from E. coli) PubMed
  • KEGG entry: [2]
  • E.C. number:

Additional information

Expression and regulation

  • Sigma factor:
  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 209 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References