yjbI

168

truncated hemoglobin, NO protection, prevents oxidative aggregation of cell-surface proteins

locus: BSU_11560

Molecular weight: 14.98 kDa

pI: 5.17

Protein length: 132 aa

Gene length: 399 bp

function: prevents oxidative aggregation of cell-surface proteins

product: protein hydroperoxide peroxidase

essential: no

synonyms: yjbI

List of homologs in different organisms, belongs to COG2346 (Galperin et al., 2021)

Gene

Coordinates: 1,234,510  1,234,908

Phenotypes of a mutant

• lowered biofilm water repellence, due to cross-linked aggregation of the biofilm matrix protein [protein|BF97457E986656E4A9FE7A858F5BDF1759850D5C|tasA] [pubmed|36125244]

The protein

Catalyzed reaction/ biological activity

• binds sulfide with high affinity [Pubmed|20102180]
• removes hydroperoxide groups from oxidised proteins [pubmed|36125244]

Protein family

• truncated hemoglobin family (single member, according to UniProt)

Structure

• [PDB|1UX8]
• [AF|O31607]

Kinetic information

• K(D) for hydrogen sulfide = 5.0x10(6) M-1 [Pubmed|20102180]

[wiki|Localization]

• cell surface [pubmed|36125244]

Expression and Regulation

Operons

• genes: [gene|948BD57CC45915C7CDDB8EF168287533FC514B7C|yjbI]-[gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH]

  description: [Pubmed|17293416]

  regulation

  • induced by cell wall stress ([protein|2C6386E9A63F410558D168798D077DF91590F454|spx]) [pubmed|29271514]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [pubmed|29271514], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

  additional information

  • A [protein|search|ncRNA] is predicted between [gene|138DC46C976BAA18893B70DF040BA1BB2FA0A77C|yizD] and [gene|87124945A1CBF7990856FBEB9EAD25096DAC0868|spxH] [PubMed|20525796]

Biological materials

Mutant

• MGNA-B155 (yjbI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1154 NBRP B. subtilis, Japan]
• 1A864 ( ''yjbI''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A864&Search=1A864 BGSC]
• BKE11560 ([gene|948BD57CC45915C7CDDB8EF168287533FC514B7C|yjbI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE11560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTGACTACTCCTTTAA,  downstream forward: _UP4_TCTTGACAAACTATCAGCAT
• BKK11560 ([gene|948BD57CC45915C7CDDB8EF168287533FC514B7C|yjbI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK11560 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTTGACTACTCCTTTAA,  downstream forward: _UP4_TCTTGACAAACTATCAGCAT

References

• 17293416,19899166,20102180,22759230,26001125,29271514,36125244