frlO

168

aminosugar ABC transporter (binding protein)

Locus: BSU_32600

Molecular weight: 47.86 kDa

Isoelectric point: 5.67

Protein length: 422 aa

Gene length: 1269 bp

Function: uptake of sugar amines

Product: aminosugar ABC transporter (binding protein)

Essential: no

Synonyms: frlO, yurO

Genomic Context

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List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)

Gene

Coordinates: 3,349,761  3,351,029

The protein

Protein family

• Bacterial solute-binding protein 1 family (according to UniProt)

Structure

• 3ZKK (PDB) (from Bifidobacterium animalis, 26% identity) PubMed
• O32156 (AlphaFold)

Localization

• membrane associated (via FrlM-FrlN) PubMed

Expression and Regulation

Operons

• Genes: frlB-frlO-frlN-frlM-frlD-frlP

  Description: PubMed

  Regulation

  • '' FrlB'': repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced in the absence of RnpM PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, PubMed, in codY regulon
  • FrlR: repression, PubMed, in frlR regulon
  • RnpM: unknown, PubMed, in rnpM regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the frlB-frlO-frlN-frlM-frlD-frlP operon is strongly downregulated in a cshA mutant PubMed

Biological materials

Mutant

• MGNA-A561 (yurO::erm), available at the NBRP B. subtilis, Japan
• BKE32600 (frlO::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGGGTTGGCCTCCTCTG,  downstream forward: _UP4_TAAATCAAAGAAAAGGTGAA
• BKK32600 (frlO::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAAGGGTTGGCCTCCTCTG,  downstream forward: _UP4_TAAATCAAAGAAAAGGTGAA

References

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