frlD

168

fructosamine kinase

Locus: BSU_32570

Molecular weight: 30.83 kDa

Isoelectric point: 4.91

Protein length: 284 aa

Gene length: 855 bp

Function: metabolism of sugar amines

Product: fructosamine kinase

Essential: no

Synonyms: frlD, yurL

Genomic Context

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List of homologs in different organisms, belongs to COG0524 (Galperin et al., 2021)

Gene

Coordinates: 3,347,051 → 3,347,905

The protein

Protein family

• carbohydrate kinase PfkB family (according to UniProt)

Structure

• 1TYY (PDB) (from Salmonella typhimurium, 24% identity) PubMed
• O32153 (AlphaFold)

Expression and Regulation

Operons

• Genes: frlB-frlO-frlN-frlM-frlD-frlP

  Description: PubMed

  Regulation

  • '' FrlB'': repressed during growth in the presence of branched chain amino acids (CodY) PubMed
  • induced in the absence of RnpM PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, PubMed, in codY regulon
  • FrlR: repression, PubMed, in frlR regulon
  • RnpM: unknown, PubMed, in rnpM regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • the frlB-frlO-frlN-frlM-frlD-frlP operon is strongly downregulated in a cshA mutant PubMed

Biological materials

Mutant

• MGNA-A558 (yurL::erm), available at the NBRP B. subtilis, Japan
• BKE32570 (ΔfrlD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCAATTTCATAGCGCTTC,  downstream forward: _UP4_TAAGCAGTGTCTCGTTTTTT
• BKK32570 (ΔfrlD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_AATCAATTTCATAGCGCTTC,  downstream forward: _UP4_TAAGCAGTGTCTCGTTTTTT

References

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