disA
168
DNA integrity scanning protein, diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA replication during B. subtilis spore outgrowth until the germinating spores genome is free of damage
Locus
BSU_00880
Molecular weight
40.58 kDa
Isoelectric point
5.57
Function
control of sporulation initiation, synthesis of c-di-AMP
Product
DNA integrity scanning protein, has diadenylate cyclase activity
Essential
no
E.C.
2.7.7.85
Synonyms
disA, yacK
Outlinks
Genomic Context
Categories containing this gene/protein
List of homologs in different organisms, belongs to COG1623 (Galperin et al., 2021)
This gene is a member of the following regulons
Gene
Coordinates
107,476 108,558
Phenotypes of a mutant
The protein
Catalyzed reaction/ biological activity
binds to abasic DNA lesions PubMed
Protein family
DisA family (single member, according to UniProt)
DAC domain (aa 11-149) (according to UniProt)
Mn2+ PubMed
Structure
3C23 (PDB) (from Thermotoga maritima, 42.9% identity) PubMed
Effectors of protein activity
the presence of potassium results in enhanced activity PubMed
forms discrete globular foci in germinating spres that colocalize with the nucleoid PubMed
Expression and Regulation
Operons
Description
Regulation
expressed during germination and spore outgrowth PubMed
Regulatory mechanism
Sigma factors
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Biological materials
Mutant
MGNA-B932 (disA::erm), available at the NBRP B. subtilis, Japan
GP2142 (ΔdisA::tet), available in Jörg Stülke's lab
GP2222 (cdaA::cat cdaS::ermC disA::tet), available in Jörg Stülke's lab, the mutant is only viable on minimal medium at low potassium concentration PubMed
BKE00880 (disA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA, downstream forward: _UP4_TGATTTCGGTTAAAACCTTA
BKK00880 (disA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA, downstream forward: _UP4_TGATTTCGGTTAAAACCTTA
GP2715 (ΔdisA::spc), available in Jörg Stülke's lab
GP2752 (ΔdisA::cat), available in Jörg Stülke's lab
GP2782 (ΔdisA::kan), available in Jörg Stülke's lab
Expression vectors
IPTG inducible expression of His-disA in E. coli: pGP2563 (in pET19b), available in Jörg Stülke's lab PubMed
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
References
Reviews
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Original Publications
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Time of last update: 2025-04-02 03:47:02
Author of last update: Jstuelk