disA

disA
168

DNA integrity scanning protein, diadenylate cyclase, delays sporulation in the case of chromosome damage, the DisA-dependent checkpoint arrests DNA replication during B. subtilis spore outgrowth until the germinating spores genome is free of damage

Locus
BSU_00880
Molecular weight
40.58 kDa
Isoelectric point
5.57
Protein length
Gene length
Function
control of sporulation initiation, synthesis of c-di-AMP
Product
DNA integrity scanning protein, has diadenylate cyclase activity
Essential
no
E.C.
2.7.7.85
Synonyms
disA, yacK

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1623 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
107,476  108,558
Phenotypes of a mutant
a cdaA disA double mutant or cdaA cdaS disA triple mutant is not viable on complex medium; however, the mutant grows at low potassium concentration (0.1 mM) PubMed
reduced spore survival after infrared exposure PubMed
altered morphology on MSgg medium PubMed
plant root colonization defect PubMed
The protein
Catalyzed reaction/ biological activity
synthesis of c-di-AMP from two molecules of ATP PubMed
binds to abasic DNA lesions PubMed
binds RecA to inhibit its activity PubMed
limits RecG activities at stalled or reversed replication forks PubMed
limits RuvA-RuvB-mediated fork remodeling and RecU-mediated HJ cleavage to provide time for damage removal and replication restart in order to preserve genome integrity PubMed
Protein family
DisA family (single member, according to UniProt)
contains a DAC domain for the synthesis of c-di-AMP PubMed
DAC domain (aa 11-149) (according to UniProt)
Mn2+ PubMed
Structure
3C23 (PDB) (from Thermotoga maritima, 42.9% identity) PubMed
Effectors of protein activity
RadA is an inhibitor of DisA activity PubMed
the presence of potassium results in enhanced activity PubMed
see a video showing the movement of DisA in the cell (in real time) PubMed
forms discrete globular foci in germinating spres that colocalize with the nucleoid PubMed
forms rapidly moving focus that pauses at RecA-mediated recombination intermediates upon induction of DNA damage during spore development PubMed
Expression and Regulation
Operons
Description
Regulation
expressed during germination and spore outgrowth PubMed
Regulatory mechanism
CtsR: repression, PubMed, in ctsR regulon
Spx: activation, PubMed, in spx regulon
Sigma factors
SigM: sigma factor, PubMed, in sigM regulon
SigA: sigma factor, PubMed, in sigA regulon
SigB: sigma factor, PubMed PubMed, in sigB regulon
SigF: sigma factor, PubMed, in sigF regulon
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ctsRdisA

2025-03-17 18:53:30

Bzhu

158

b7066b5ca5004b095502d71420c469e9ee925bfa

163769A1708F45F8ABE642374A97FA8A00293529

Genes
Description
Regulation
expressed during germination and spore outgrowth PubMed
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disA

2025-03-15 21:10:38

ghost

129

83f82fe93a5d7fc7c4cde98212b1c8cac4b0a273

3EB963FA933A46D0CD3DFCC171D4F5D901936AC9

Biological materials
Mutant
MGNA-B932 (disA::erm), available at the NBRP B. subtilis, Japan
GP2142 (ΔdisA::tet), available in Jörg Stülke's lab
BKG2 (radA-disA::spc), available  in Jörg Stülke's lab
1A939 (disA::tet), PubMed, available at BGSC
GP2222 (cdaA::cat cdaS::ermC disA::tet), available in Jörg Stülke's lab, the mutant is only viable on minimal medium at low potassium concentration PubMed
BKE00880 (disA::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA,  downstream forward: _UP4_TGATTTCGGTTAAAACCTTA
BKK00880 (disA::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CCCTTTTTTCTCTTTTTCCA,  downstream forward: _UP4_TGATTTCGGTTAAAACCTTA
GP2715 (ΔdisA::spc), available in Jörg Stülke's lab
GP2752 (ΔdisA::cat), available in Jörg Stülke's lab
GP2782 (ΔdisA::kan), available in Jörg Stülke's lab
Expression vectors
IPTG inducible expression of His-disA in E. coli: pGP2563 (in pET19b), available in Jörg Stülke's lab PubMed
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab
References
Reviews
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Original Publications
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2B091CCEE9E34D659771E39B1FC9050A145048AB

Page visits: 11575

Time of last update: 2025-04-02 03:47:02

Author of last update: Jstuelk