bshB1

168

N-acetylglucosamine-malate deacetylase, involved in bacillithiol synthesis (major enzyme)

Locus: BSU_22470

Molecular weight: 24.66 kDa

Isoelectric point: 6.34

Protein length: 236 aa

Gene length: 711 bp

Function: biosynthesis of bacillithiol

Product: N-acetylglucosamine-malate deacetylase

Essential: no

Synonyms: bshB1, jojG, ypjG

Genomic Context

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List of homologs in different organisms, belongs to COG2120 (Galperin et al., 2021)

Gene

Coordinates: 2,358,208  2,358,918

The protein

Catalyzed reaction/ biological activity

• (S)-malyl N-acetyl-α-D-glucosaminide + H₂O --> (S)-malyl α-D-glucosaminide + acetate (according to UniProt)

Protein family

• PIGL family (with BshB2, according to UniProt)

Cofactors

• Zn²⁺ PubMed

Structure

• 6ULL (PDB) (complex with substrate analogue) PubMed
• 6P2T (PDB) (complex with citrate) PubMed
• 2IXD (PDB) (from B. cereus, 62% identity) PubMed
• P42981 (AlphaFold)

Paralogous protein(s)

• BshB2

Expression and Regulation

Operons

• Genes: ypjD-dapB-mgsA-bshB1-bshA-cca-birA

  Description: PubMed

  Regulation

  • induced by diamide stess (thiol depletion) (Spx) PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A409 (ypjG::erm), available at the NBRP B. subtilis, Japan
• bshB1 null mutant and ''bshB1 / bshB2'' double null mutant available in John Helmann lab
• GP87 (ypjG::pX2(cat)), available in Jörg Stülke's lab
• BKE22470 (bshB1::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT,  downstream forward: _UP4_CTTGATCATGATGTGCTTGG
• BKK22470 (bshB1::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GGCGCCAAAAGCAAGAACGT,  downstream forward: _UP4_CTTGATCATGATGTGCTTGG

References

Reviews

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Original Publications

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