bshA

168

L-malic acid glycosyltransferase, involved in bacillithiol synthesis

Locus: BSU_22460

Molecular weight: 41.82 kDa

Isoelectric point: 6.15

Protein length: 377 aa

Gene length: 1134 bp

Function: biosynthesis of bacillithiol

Product: L-malic acid glycosyltransferase

Essential: no

Synonyms: bshA, jojH, ypjH

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0438 (Galperin et al., 2021)

Gene

Coordinates: 2,357,078  2,358,211

Phenotypes of a mutant

• impaired growth in minimal medium, reduced activity of enzymes containing FeS cluster PubMed
• sensitivity to superoxide (paraquate) stress PubMed

The protein

Catalyzed reaction/ biological activity

• UDP-GlcNAc + L-malate --> (S)-malyl N-acetyl-α-D-glucosaminide + H⁺ + UDP PubMed; also uses D-malate as a substrate, but with much lower affinity PubMed

Protein family

• Glycosyltransferase 1 family (according to UniProt)
• Glycosyltransferase 4 subfamily (according to UniProt)

Structure

• 5D00 (PDB) PubMed
• P42982 (AlphaFold)

Effectors of protein activity

• subject to feedback inhibition by bacillithiol PubMed

Expression and Regulation

Operons

• Genes: ypjD-dapB-mgsA-bshB1-bshA-cca-birA

  Description: PubMed

  Regulation

  • induced by diamide stess (thiol depletion) (Spx) PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A410 (ypjH::erm), available at the NBRP B. subtilis, Japan
• bshA::mls available in John_Helmann lab
• GP88 (bshA::pX2(cat)), available in Jörg Stülke's lab
• BKE22460 (bshA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTCATTGTTCGCCCCCAAG,  downstream forward: _UP4_GATTTAGCAGAACCGGAGTG
• BKK22460 (bshA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TCTCATTGTTCGCCCCCAAG,  downstream forward: _UP4_GATTTAGCAGAACCGGAGTG

References

Reviews

Loading

Original Publications

Loading