lysC

168

aspartokinase II (alpha and beta subunits)

Locus: BSU_28470

Molecular weight: 43.65 kDa

Isoelectric point: 4.64

Protein length: 408 aa

Gene length: 1227 bp

Function: biosynthesis of lysine

Product: aspartokinase II (alpha and beta subunits)

Essential: no

E.C.: 2.7.2.4

Synonyms: lysC, ask, aecA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0527 (Galperin et al., 2021)

Gene

Coordinates: 2,909,520 2,910,746

The protein

Catalyzed reaction/ biological activity

• ATP + L-aspartate --> 4-phospho-L-aspartate + ADP (according to UniProt)

Protein family

• aspartokinase family (with DapG and ThrD, according to UniProt)

Domains

• two C-terminal ACT domains (aa 264-337, and aa 343-408) (according to UniProt)

Structure

• 2RE1 (PDB) (from Neisseria meningitidis mc58, 40% identity, 58% similarity)
• P08495 (AlphaFold)

Effectors of protein activity

• feedback inhibition by lysine PubMed

Paralogous protein(s)

• DapG

Localization

• cytoplasm (according to UniProt)

Additional information

• subject to Clp-dependent proteolysis upon glucose starvation PubMed, also degraded upon ammonium or amino acid starvation PubMed
• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: lysC

  Description: PubMed

  Regulation

  • expression activated by glucose (5.4 fold) PubMed

  Regulatory mechanism

  • L-box: RNA switch, via a riboswitch, in L-box

Biological materials

Mutant

• BKE28470 (lysC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG
• BKK28470 (lysC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTATTACCACCCTTTAC, downstream forward: _UP4_TAATGACAATCAAAAAGGCG

References

Reviews

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Research papers

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The L-box riboswitch

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