gabT

168

gamma-aminobutyrate transaminase, general stress protein

Locus: BSU_03900

Molecular weight: 47.09 kDa

Isoelectric point: 5.3

Protein length: 436 aa

Gene length: 1308 bp

Function: utilization of gamma-amino butyric acid

Product: gamma-aminobutyrate transaminase

Essential: no

E.C.: 2.6.1.19

Synonyms: gabT, ycnG

Genomic Context

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List of homologs in different organisms, belongs to COG0160 (Galperin et al., 2021)

Gene

Coordinates: 441,571 → 442,881

Phenotypes of a mutant

• growth defect in the presence of gamma-aminobutyrate  PubMed
• no growth with gamma-aminobutyrate as single carbon source  PubMed

The protein

Catalyzed reaction/ biological activity

• 4-aminobutanoate + 2-oxoglutarate --> succinate semialdehyde + L-glutamate (according to UniProt)
• (S)-3-amino-2-methylpropanoate + 2-oxoglutarate --> 2-methyl-3-oxopropanoate + L-glutamate (according to UniProt)
• the protein was reported to be involved in norspermidine production and biofilm disassembly PubMed; however, this is not the case PubMedand the original paper has been retracted

Protein family

• Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 1SF2 (PDB) (from E. coli, 45% identity) PubMed
• P94427 (AlphaFold)

Paralogous protein(s)

• RocD, ArgD
• YhxA, (32.8%)

Expression and Regulation

Operons

• Genes: gabT-gabD

  Description: PubMed

  Regulation

  • ''gabD'': induced by stress (SigB) PubMed

  Regulatory mechanism

  • GabR: activation, PubMed, in gabR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C068 (ycnG::erm), available at the NBRP B. subtilis, Japan
• BKE03900 (ΔgabT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAATATCCCCCTGTC,  downstream forward: _UP4_TAATCATTGGAAAGAAAATG
• BKK03900 (ΔgabT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGAATATCCCCCTGTC,  downstream forward: _UP4_TAATCATTGGAAAGAAAATG

Labs working on this gene/protein

• Linc Sonenshein, Tufts University, Boston, MA, USA Homepage

References

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