tseB

168

cell shape control via PBP 2A, required for efficient cell wall elongation during exponential phase and spore outgrowth, tetracycline sensitivity suppressor of ezrA

Locus: BSU_22380

Molecular weight: 17.75 kDa

Isoelectric point: 9.24

Protein length: 161 aa

Gene length: 486 bp

Function: control of PBP 2A

Product: regulator of PBP 2A

Essential: no

Synonyms: tseB, ypmB

Genomic Context

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List of homologs in different organisms, belongs to COG5353 (Galperin et al., 2021)

Gene

Coordinates: 2,348,864 → 2,349,349

Phenotypes of a mutant

• resistant to moenomycin PubMed
• shorter and wider than wild type cells PubMed
• PBP 2A over expression is toxic in the mutant PubMed
• inactivation suppresses the essentiality of mbl PubMed

The protein

Structure

• 2GU3 (PDB)
• P54396 (AlphaFold)

Localization

• cell membrane(according to UniProt)

Expression and Regulation

Operons

• Genes: panB-panC-panD-dinG-ypmA-tseB-aspB

  Description: PubMed

• Genes: ypmA-tseB-aspB

  Description: PubMed

• Genes: tseB-aspB

  Description: PubMed

  Regulation

  • constitutive

Biological materials

Mutant

• MGNA-A441 (ypmB::erm), available at the NBRP B. subtilis, Japan
• BKE22380 (ΔtseB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCTTTTTTTCTCATCTTCT,  downstream forward: _UP4_TAAGACGAATTAGGGGGAGT
• BKK22380 (ΔtseB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCTTTTTTTCTCATCTTCT,  downstream forward: _UP4_TAAGACGAATTAGGGGGAGT

References

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