aspB

168

aspartate transaminase

Locus: BSU_22370

Molecular weight: 42.93 kDa

Isoelectric point: 5.3

Protein length: 393 aa

Gene length: 1182 bp

Function: biosynthesis of aspartate

Product: aspartate transaminase

Essential: no

E.C.: 2.6.1.1

Synonyms: aspB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

Gene

Coordinates: 2,347,660 2,348,841

Phenotypes of a mutant

• auxotrophic for aspartate and asparagine PubMed, the auxotrophy for aspartate can be bypassed by derepression of the ansA-ansB operon (inactivation of ansR) PubMed
• lyses on DSM dporulation medium due to the inhibition of GltT-mediated Asp transport by glutamate and resulting depletion of meso-diaminopimelate PubMed

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + L-aspartate --> L-glutamate + oxaloacetate (according to UniProt)

Protein family

• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP

Structure

• 1J32 (PDB) (from Phormidium lapideum, 45% identity, 66% similarity)
• P53001 (AlphaFold)

Paralogous protein(s)

• AlaT, PatA

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: panB-panC-panD-dinG-ypmA-tseB-aspB

  Description: PubMed

• Genes: ypmA-tseB-aspB

  Description: PubMed

• Genes: tseB-aspB

  Description: PubMed

  Regulation

  • constitutive

Biological materials

Mutant

• GP4250 (ΔaspB::neo), available in Jörg Stülke's lab
• BKE22370 (aspB::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT
• BKK22370 (aspB::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAACTTGAACTCCCCCTAAT, downstream forward: _UP4_TAACAGATCAAAAAGCGGCT

References

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