sigM

sigM
168

RNA polymerase ECF-type sigma factor SigM, required to prioritize carrier lipid (undecaprenyl-phosphate) utilization for peptidoglycan synthesis

Locus
BSU_09520
Molecular weight
19.26 kDa
Isoelectric point
6.77
Protein length
Gene length
Function
adaptation to inhibitors of peptidoglycan synthesis
Essential
no
Synonyms
sigM, yhdM

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG1595 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
1,029,577  1,030,068
Phenotypes of a mutant
sigM is essential for growth and survival in nutrient broth (NB) containing 1.4 M NaCl PubMed
sigM mutants form aberrantly shaped cells, which swell and lyse spontaneously during growth in NB medium containing increased levels (0.35-0.7 M) of a wide range of different salts PubMed
increased sensitivity towards beta-lactam antibiotics PubMed
a ''sigM murJ'' double mutant is not viable (due to the lack of both lipid II flippases) PubMed
poor growth of a bcrC sigM double mutant PubMed
The protein
Protein family
Sigma-70 factor family (according to UniProt)
ECF subfamily (according to UniProt)
Structure
5WUQ (PDB) (SigW in complex with the cytoplasmic domain of RsiW, 32% identity) PubMed
Effectors of protein activity
expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
shortage of available bactoprenol causes SigM activation PubMed
antibiotics that block UndP recycling result in activation of SigM PubMed
SigM is released from the YhdL-YhdK complex upon undecaprenyl-phosphate limitation PubMed
Additional information
Expression of the SigM regulon in increased in ''ugtP'' mutants PubMed
information on binding sites can be found in the PRODORIC2 database
Expression and Regulation
Operons
Description
Regulation
induced by glucose PubMed
Sigma factors
SigA: sigma factor, PubMed, in sigA regulon
SigM: sigma factor, PubMed, in sigM regulon
Additional information
expression of CsbB in the absence of YfhO causes constitutive activation of SigM PubMed
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sigMyhdK

2024-12-20 02:52:17

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3F70EAF5AFE9E64A7150341483663D9A7347A835

Biological materials
Mutant
1A906 (sigM::kan), PubMed, available at BGSC
BP97 (Δ(sigM-yhdL-yhdK)::aphA3), available in Fabian Commichau's lab
BP129 (ΔsigM::aphA3), available in Fabian Commichau's lab
BKE09520 (sigM::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACTGCCTTTTCTCCCCTCT,  downstream forward: _UP4_AAAGCACTTTATAATAGAGG
BKK09520 (sigM::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CACTGCCTTTTCTCCCCTCT,  downstream forward: _UP4_AAAGCACTTTATAATAGAGG
Labs working on this gene/protein
John Helmann, Cornell University, USA Homepage
References
Reviews
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Other original publications
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081DF3EE9FA56209D648C7677188C61CE3AA8E41

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Time of last update: 2024-12-20 07:46:37

Author of last update: Jstuelk