yocH

168

peptidoglycan hydrolase (N-acetylglucosamidase)

Locus: BSU_19210

Molecular weight: 30.03 kDa

Isoelectric point: 8.37

Protein length: 287 aa

Gene length: 864 bp

Function: cell wall turnover

Product: peptidoglycan hydrolase

Essential: no

Synonyms: yocH

Genomic Context

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List of homologs in different organisms, belongs to COG3584 (Galperin et al., 2021)

Gene

Coordinates: 2,092,899 → 2,093,762

The protein

Domains

• contains two N-acetylglucosamine-polymer-binding LysM domains PubMed
• 2 LysM domains (aa 26-69, aa 78-121) (according to UniProt)

Structure

• 4WLK (PDB) (YuiC, corresponds to aa 155 ... 286 of YocH, 34% identity) PubMed
• O34669 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: yocH

  Description: PubMed

  Regulation

  • expressed under conditions that trigger sporulation (Spo0A) PubMed

  Regulatory mechanism

  • Spo0A: activation, PubMed, in spo0A regulon
  • AbrB: repression, PubMed, in abrB regulon
  • WalR: activation, PubMed, in walR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: bsrB-recQ-yocH

  Description: PubMed

Biological materials

Mutant

• MGNA-A321 (yocH::erm), available at the NBRP B. subtilis, Japan
• AH023 knock-out mutant (kan), available in Kevin Devine's and Jörg Stülke's labs PubMed
• BKE19210 (''yocH::erm'', available in the BGSC and in Jörg Stülke's lab) PubMed
• BKE19210 (ΔyocH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG,  downstream forward: _UP4_TAATAATATAGATTGTGATA
• BKK19210 (ΔyocH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAGTAAATCCTCCCTTG,  downstream forward: _UP4_TAATAATATAGATTGTGATA

Expression vectors

• pGP1691, expression of yocH-Strep in B. subtilis, in pGP382, available in Jörg Stülke's lab
• pGP1693, expression of yocH-Strep in E. coli, in pGP574, available in Jörg Stülke's lab

Labs working on this gene/protein

• Kevin Devine

References

Reviews

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Original Publications

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