tapA

168

primer for TasA oligomerization

Locus: BSU_24640

Molecular weight: 28.92 kDa

Isoelectric point: 6.68

Protein length: 253 aa

Gene length: 762 bp

Function: biofilm formation

Product: primer for TasA oligomerization

Essential: no

Synonyms: tapA, yqhD, yqxM

Genomic Context

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List of homologs in different organisms

Gene

Coordinates: 2,554,486  2,555,247

Phenotypes of a mutant

• no biofilm formation PubMed

The protein

Catalyzed reaction/ biological activity

• binds TasA to initiate TasA oligomerization PubMed

Structure

• 6HQC (PDB) (aa 75 ... 190) PubMed
• 6QAY (PDB) (NMR structure) PubMed
• 8AIF (PDB) (aa 71 ... 190) PubMed
• P40949 (AlphaFold)

Localization

• attached to the cell surface (on the outside of the cell), associated with peptidoglycan  PubMed
• secretion requires SipW PubMed

Expression and Regulation

Operons

• Genes: tapA-sipW-tasA

  Description: PubMed

  Regulation

  • repressed by casamino acids PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon
  • SinR: repression, PubMed, in sinR regulon
  • RemA: activation, PubMed, in remA regulon
  • LutR: activation, PubMed, in lutR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • induction by sequestration of SinR by SinI or SlrA PubMed or by SlrR PubMed
  • expression of the operon is localized to a ring near the periphery of the biofilm PubMed

Biological materials

Mutant

• MGNA-C429 (yqhD::erm), available at the NBRP B. subtilis, Japan
• BKE24640 (tapA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTTACCTCCTGTAAA,  downstream forward: _UP4_AGCAATGAAGCTGATCAGTA
• BKK24640 (tapA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCTTACCTCCTGTAAA,  downstream forward: _UP4_AGCAATGAAGCTGATCAGTA

LacZ fusion

• pGP1926 (in pAC6), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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