rpoE

168

RNA polymerase delta subunit, affects the regulation of RNA polymerase by the concentration of the initiating nucleoside triphosphate (iNTP)

Locus: BSU_37160

Molecular weight: 20.25 kDa

Isoelectric point: 3.65

Protein length: 173 aa

Gene length: 522 bp

Function: transcription

Product: RNA polymerase delta subunit

Essential: no

E.C.: 2.7.7.6

Synonyms: rpoE

Genomic Context

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List of homologs in different organisms, belongs to COG3343 (Galperin et al., 2021)

Gene

Coordinates: 3,812,542  3,813,063

Phenotypes of a mutant

• RpoE is essential for cell survival when facing a competing strain in changing environment PubMed

The protein

Catalyzed reaction/ biological activity

• binds to DNA immediately upstream of the promoter element at A-rich sequences on the abrB and rrnB1 promoters and facilitates open complex formation PubMed
• represses expression of ''spo0B'' PubMed

Protein family

• rpoE family (single member, according to UniProt)

Domains

• HARE-HTH domain (aa 15-81) (according to UniProt)

Structure

• 2M4K (PDB) (full-length protein) PubMed
• 2KRC (PDB) (N-terminal domain) PubMed
• 6ZFB (PDB) (RNA polymerase complex) PubMed
• P12464 (AlphaFold)

Localization

• closely associated with RNA polymerase involved in transcribing both mRNA and rRNA operons PubMed

Expression and Regulation

Operons

• Genes: rpoE-pyrG

  Description: PubMed

  Regulation

  • the mRNA is processed between rpoE and pyrG by RNase Y, this requires the RicA-RicF-RicT complex PubMed

• Genes: rpoE

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

• Genes: fadF-acdA-rpoE

  Description: PubMed

  Regulation

  • repressed in the absence of long-chain fatty acids (FadR) PubMed

  Regulatory mechanism

  • FadR: repression, PubMed, in fadR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Additional information

• present at equimolar levels with RNA polymerase PubMed

Biological materials

Mutant

• GP3216 (rpoE::aphA3), available in Jörg Stülke's lab
• BKE37160 (rpoE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGGTCGGACACTCCCTTT,  downstream forward: _UP4_TAGTATCAGATTTCATCTTC
• BKK37160 (rpoE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGGTCGGACACTCCCTTT,  downstream forward: _UP4_TAGTATCAGATTTCATCTTC

LacZ fusion

• BP1145: Promotor-lacZ fusion, available at Jörg Stülke's and Fabian Commichau's labs

Labs working on this gene/protein

• Arthur Aronson, Purdue University, West Lafayette, USA homepage

References

Reviews

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Original Publications

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