maf

168

nucleotide pyrophosphatase

Locus: BSU_28050

Molecular weight: 21.15 kDa

Isoelectric point: 5.71

Protein length: 189 aa

Gene length: 570 bp

Function: unknown

Product: nucleotide pyrophosphatase

Essential: no

Synonyms: maf

Genomic Context

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List of homologs in different organisms, belongs to COG0424 (Galperin et al., 2021)

Gene

Coordinates: 2,862,572  2,863,141

The protein

Catalyzed reaction/ biological activity

• nucleotide pyrophosphatase PubMed
• dTTP + H₂O --> diphosphate + dTMP + H⁺ (according to UniPort)
• H₂O + UTP --> diphosphate + H⁺ + UMP (according to UniPort)
• CTP + H₂O --> CMP + diphosphate + H⁺ (according to UniPort)
• H₂O + ψ-UTP --> diphosphate + H⁺ + ψ-UMP (according to UniPort)
• 5-methyl-CTP + H₂O --> 5-methyl-CMP + diphosphate + H⁺ (according to UniPort)
• 5-methyl-UTP + H₂O --> 5-methyl-UMP + diphosphate + H⁺ (according to UniPort)

Protein family

• maf family (single member, according to UniProt)

Cofactors

• binds phosphorylated compounds (UTP, IMP, IDP, GDP and others) in in vitro experiments

Structure

• 1EX2 (PDB) PubMed
• Q02169 (AlphaFold)

Localization

• cytosolic protein PubMed

Expression and Regulation

Operons

• Genes: maf-radC-mreB-mreC-mreD-minC-minD

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigM: sigma factor, promoter p2 within Maf PubMed, in sigM regulon
  • SigA: sigma factor, promoter p1, upstream of Maf PubMed, in sigA regulon

Biological materials

Mutant

• BKE28050 (maf::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTCGTCATCCTTTCAG,  downstream forward: _UP4_TGACGGTTCTATAAACGGGA

References

Original Publications

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