opuBB

168

choline and arsenocholine ABC transporter (membrane protein)

Locus: BSU_33720

Molecular weight: 23.02 kDa

Isoelectric point: 9.74

Protein length: 217 aa

Gene length: 654 bp

Function: compatible solute transport

Product: choline and arsenocholine ABC transporter (membrane protein)

Essential: no

Synonyms: opuBB, proW

Genomic Context

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List of homologs in different organisms, belongs to COG1174 (Galperin et al., 2021)

Gene

Coordinates: 3,461,435  3,462,088

The protein

Catalyzed reaction/ biological activity

• uptake of choline and arsenocholine PubMed

Protein family

• Binding-protein-dependent transport system permease family (according to UniProt)
• CysTW subfamily (according to UniProt)

Domains

• ABC transmembrane type-1 domain (aa 19-198) (according to UniProt)

Structure

• Q45461 (AlphaFold)

Paralogous protein(s)

• OpuCB

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: opuBA-opuBB-opuBC-opuBD

  Description: PubMed

  Regulation

  • induced by choline (GbsR) PubMed

  Regulatory mechanism

  • GbsR: repression, (transcriptional roadblock) PubMed, in gbsR regulon
  • OpcR: repression, (sterical interference with RNA polymerase access) PubMed, in opcR regulon
  • RemA: activation, PubMed, in remA regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • An antisense RNA is predicted for'opuBD' PubMed

Biological materials

Mutant

• BKE33720 (opuBB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTTATCGCCCCTCCC,  downstream forward: _UP4_GAATTGTCATAAGGAGGCGG
• BKK33720 (opuBB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTTATCGCCCCTCCC,  downstream forward: _UP4_GAATTGTCATAAGGAGGCGG

References

Reviews

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Original Publications

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