acoB

acoB
168

acetoin dehydrogenase E1 component (TPP-dependent beta subunit)

Locus
BSU_08070
Molecular weight
36.69 kDa
Isoelectric point
4.4
Protein length
Gene length
Function
acetoin utilization
Product
acetoin dehydrogenase E1 component (TPP-dependent beta subunit)
Essential
no
Synonyms
acoB, yfjJ

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0022 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
880,007  881,035
Phenotypes of a mutant
defective in the the ability to support the growth of Synechococcus leopoliensis CCAP1405/1 on agar media PubMed
The protein
Structure
3DUF (PDB) (PDH from Geobacillus stearothermophilus, 43% identity) PubMed
Paralogous protein(s)
Membrane-proximal (Spotty) PubMed
Expression and Regulation
Operons
Description
Regulation
induced by acetoin (AcoR) PubMed
Regulatory mechanism
CcpA: repression, PubMed, in ccpA regulon
AcoR: activation, (interaction with SigL-containing RNA polymerase) [ PubMed|11274109], in acoR regulon
Fnr: indirect positive regulation, in fnr regulon
Sigma factors
SigL: sigma factor, PubMed, in sigL regulon
Additional information
the mRNA is substantially stabilized upon depletion of RNase Y PubMed
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acoAacoL

2024-12-03 14:11:04

Bzhu

113

13d8d82e494fdc730b04ec27f52013adc2c6a3b0

0F3039221AB7F088A4D6093D7D4525D99014DE74

Additional information
the mRNA is very stable (> 15 min) PubMed
Biological materials
Mutant
MGNA-C281 (acoB::erm), available at the NBRP B. subtilis, Japan
BKE08070 (acoB::erm  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGCTTATGACTCTCGCCA,  downstream forward: _UP4_GCATTGAATTAAAGGGGGGA
BKK08070 (acoB::kan  trpC2) available at BGSCPubMed, upstream reverse: _UP1_CATGCTTATGACTCTCGCCA,  downstream forward: _UP4_GCATTGAATTAAAGGGGGGA
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
Labs working on this gene/protein
Michel Debarbouille, Pasteur Institute, Paris, France Homepage
References
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Time of last update: 2024-12-18 15:50:30

Author of last update: LKrueger