dacB

168

sporulation-specific Class C penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase

Locus: BSU_23190

Molecular weight: 42.92 kDa

Isoelectric point: 9.73

Protein length: 382 aa

Gene length: 1149 bp

Function: control of peptide cross-linking in spore peptidoglycan

Product: penicillin-binding protein 5*, D-alanyl-D-alanine carboxypeptidase

Essential: no

Synonyms: dacB

Genomic Context

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List of homologs in different organisms, belongs to COG1686 (Galperin et al., 2021)

Gene

Coordinates: 2,423,383  2,424,531

Phenotypes of a mutant

• inactivation of ''dacB'' reduces sporulation efficiency to 19.3% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• modifies degree of cross-linking of glycan strands in peptidoglycan PubMed
• Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine (according to UniProt)

Protein family

• Peptidase S11 family (according to UniProt)

Structure

• 3MFD (PDB) (beta-lactamase superfamily domain)
• P35150 (AlphaFold)

Paralogous protein(s)

• DacF

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: dacB-spmA-spmB

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE) PubMed

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• MGNA-A098 (dacB::erm), available at the NBRP B. subtilis, Japan
• 1A744 ( dacB::cat), PubMed, available at BGSC
• 1A745 ( dacB::cat), PubMed, available at BGSC
• BKE23190 (dacB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG,  downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC
• BKK23190 (dacB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTGCTCACGTCCTTG,  downstream forward: _UP4_CTGAATGCGGCAGGCGGAGC

References

Reviews

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Original Publications

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