yfnH

168

similar to glucose-1-phosphate cytidylyltransferase

Locus: BSU_07270

Molecular weight: 28.52 kDa

Isoelectric point: 5.49

Protein length: 254 aa

Gene length: 765 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 2.7.7.33

Synonyms: yfnH

Genomic Context

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Categories containing this gene/protein

Lifestyles, Sporulation, Sporulation proteins, Sporulation proteins/ other

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG1208 (Galperin et al., 2021)

This gene is a member of the following regulons

SigE regulon, GerE regulon, SigK regulon

Gene

Coordinates: 798,469 → 799,233

The protein

Catalyzed reaction/ biological activity

α-D-glucose 1-phosphate + CTP + H⁺ --> CDP-D-glucose + diphosphate (according to UniProt)

Protein family

cytidylyltransferase family (with IspD and TagD, according to UniProt)
glucose-1-phosphate cytidylyltransferase family (single member, according to UniProt)

Structure

1TZF (PDB) (from Salmonella Typhi, 43% identity)
O06486 (AlphaFold)

Expression and Regulation

Operons

Genes: yfnH-yfnG-yfnF-yfnE-yfnD

Description: PubMed

Regulation

expressed during sporulation (SigK) PubMed

Regulatory mechanism

GerE: activation, PubMed, in gerE regulon

Sigma factors

SigK: sigma factor, PubMed, in sigK regulon
SigE: sigma factor, internal promoter in YfnE open reading frame PubMed, see DBTBS for details, in sigE regulon

Biological materials

Mutant

MGNA-C227 (yfnH::erm), available at the NBRP B. subtilis, Japan
BKE07270 (ΔyfnH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTACCCCTCATCTCT,  downstream forward: _UP4_GTATGGAAGGTATGGTGAAT
BKK07270 (ΔyfnH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTACCCCTCATCTCT,  downstream forward: _UP4_GTATGGAAGGTATGGTGAAT

References

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