spsJ

168

dTDP-glucose-4,6-dehydratase

Locus: BSU_37830

Molecular weight: 35.38 kDa

Isoelectric point: 6.29

Protein length: 315 aa

Gene length: 948 bp

Function: rhamnose biosynthesis, spore crust polysaccharide synthesis

Product: dTDP-glucose-4,6-dehydratase

Essential: no

E.C.: 4.2.1.46

Synonyms: spsJ, ipa-72d

Genomic Context

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List of homologs in different organisms, belongs to COG1088 (Galperin et al., 2021)

Gene

Coordinates: 3,884,292  3,885,239

Phenotypes of a mutant

• production of spores lacking a visible crust PubMed

The protein

Catalyzed reaction/ biological activity

• dTDP-glucose --> dTDP-4-dehydro-6-deoxyglucose PubMed

Protein family

• NAD(P)-dependent epimerase/dehydratase family (according to UniProt)

Structure

• 6BI4 (PDB) (from B. anthracis, 50% identity)
• P39630 (AlphaFold)

Paralogous protein(s)

• YfnG

Expression and Regulation

Operons

• Genes: spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE37830 (spsJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTAATTAAATATGATTTTG,  downstream forward: _UP4_AATGACCGATGAGAAGGTGA
• BKK37830 (spsJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGTAATTAAATATGATTTTG,  downstream forward: _UP4_AATGACCGATGAGAAGGTGA

References

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