spsG

168

legionaminic acid synthesis

Locus: BSU_37850

Molecular weight: 37.82 kDa

Isoelectric point: 6.56

Protein length: 339 aa

Gene length: 1020 bp

Function: legionaminic acid synthesis

Product: unknown

Essential: no

Synonyms: spsG, ipa-69d, spsH

Genomic Context

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List of homologs in different organisms, belongs to COG3980 (Galperin et al., 2021)

Gene

Coordinates: 3,886,004  3,887,023

Phenotypes of a mutant

• less hydrophobic spore surface PubMed

The protein

Catalyzed reaction/ biological activity

• required for spore crust assembly, legionaminic acid synthesis PubMed

Structure

• P39627 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE37850 (spsG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCGAAGATCCCAACATGCA,  downstream forward: _UP4_TAAAAATGCATGTAAGGAAG
• BKK37850 (spsG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGCGAAGATCCCAACATGCA,  downstream forward: _UP4_TAAAAATGCATGTAAGGAAG

References

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