spsA

168

nucleotide-sugar-dependent glycosyltransferase

Locus: BSU_37910

Molecular weight: 30.03 kDa

Isoelectric point: 5.36

Protein length: 256 aa

Gene length: 771 bp

Function: spore crust polysaccharide synthesis

Product: nucleotide-sugar-dependent glycosyltransferase

Essential: no

Synonyms: spsA, ipa-63d

Genomic Context

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List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

Gene

Coordinates: 3,892,351  3,893,121

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• reduced germination efficiency PubMed
• less hydrophobic spore surface PubMed

The protein

Protein family

• glycosyltransferase 2 family PubMed

Structure

• 1QGS (PDB) (complex with UDP) PubMed
• 1H7Q (PDB)\tPubMed
• P39621 (AlphaFold)

Paralogous protein(s)

• CgeD, Array

Expression and Regulation

Operons

• Genes: spsA-spsB-spsC-spsD-spsE-spsF-spsG-spsI-spsJ-spsK-spsL

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE) PubMed

  Regulatory mechanism

  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• BKE37910 (spsA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATCCTACACCTCCTTCT,  downstream forward: _UP4_AAAAAGCTTGGAATGGGGTG
• BKK37910 (spsA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATCCTACACCTCCTTCT,  downstream forward: _UP4_AAAAAGCTTGGAATGGGGTG

References

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