gerPF

168

spore germination protein, facilitates access of nutrient germinants to their cognate germinant receptors in spores’ inner membrane

Locus: BSU_10670

Molecular weight: 7.48 kDa

Isoelectric point: 3.62

Protein length: 75 aa

Gene length: 228 bp

Function: germination

Product: unknown

Essential: no

E.C.: null

Synonyms: gerPF, yisC

Genomic Context

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Categories containing this gene/protein

Lifestyles, Sporulation, Germination, Additional germination proteins

List of homologs in different organisms, belongs to COG5906 (Galperin et al., 2021)

This gene is a member of the following regulons

GerE regulon, SigK regulon

Gene

Coordinates: 1,148,494 → 1,148,721

The protein

Protein family

GerPA/GerPF family (according to UniProt)

Structure

O06716 (AlphaFold)

Expression and Regulation

Operons

Genes: gerPA-gerPB-gerPC-gerPD-gerPE-gerPF

Description: PubMed

Regulation

expressed during sporulation in the mother cell (SigK) PubMed

Regulatory mechanism

GerE: repression, in gerE regulon

Sigma factors

SigK: sigma factor, PubMed, in sigK regulon

Additional information

A ncRNA is predicted between 'gerPA' and 'yisI' PubMed

Biological materials

Mutant

BKE10670 (ΔgerPF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAACGACACCCTTTATC,  downstream forward: _UP4_TAAAAGCAGCCGCTCTGCAA
BKK10670 (ΔgerPF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAACGACACCCTTTATC,  downstream forward: _UP4_TAAAAGCAGCCGCTCTGCAA

References

Reviews

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Original Publications

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