cotD

168

spore coat protein (inner)

Locus: BSU_22200

Molecular weight: 8.70 kDa

Isoelectric point: 7.37

Protein length: 75 aa

Gene length: 228 bp

Function: resistance of the spore

Product: spore coat protein (inner)

Essential: no

E.C.: null

Synonyms: cotD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5879 (Galperin et al., 2021)

Gene

Coordinates: 2,332,784 → 2,333,011

The protein

Structure

• P07791 (AlphaFold)

Localization

• inner spore coat  PubMed, localization depends on SafA  PubMed

Expression and Regulation

Operons

• Genes: cotD

  Description: PubMed

  Regulation

  • expressed late during sporulation in the mother cell (SigK, SpoIIID, GerE) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon
  • GerE: activation, PubMed, in gerE regulon

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

• Genes: mrfA-mrfB-cotD

  Description: PubMed

Biological materials

Mutant

• 1S104 ( cotD::cat), PubMed, available at BGSC
• BKE22200 (ΔcotD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAAAATCCCCTTTCC,  downstream forward: _UP4_TAGTATGGAAAACGTGCAGC
• BKK22200 (ΔcotD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGATAAAATCCCCTTTCC,  downstream forward: _UP4_TAGTATGGAAAACGTGCAGC

References

Reviews

Loading

Original Publications

Loading