mcpB
168

membrane-bound chemotaxis receptor, methyl-accepting chemotaxis protein, receptor for asparagine as well as ethanol and other short-chain alcohols, acts also as pH sensor, responds to increasing pH as an attractant signal

Locus

BSU_31260

Molecular weight

71.70 kDa

Isoelectric point

5.13

Protein length

662 aaSequenceBlast

Gene length

1989 bpSequenceBlast

Function

control of chemotaxis, sensing of different compounds

Product

methyl-accepting chemotaxis protein

Essential

Synonyms

mcpB

Outlinks

BsubCyc SubtiList UniProt STRING Genoscapist PaperBLAST

Genomic Context

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Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0840 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene

Coordinates

3,210,445 → 3,212,433

The protein

Domains

Cache domain (aa 153-229) (according to UniProt)

HAMP domain (aa 304-356) (according to UniProt)

Methyl-accepting transducer domain (aa 375-611) (according to UniProt)

Structure

6S1K (PDB) (from E. coli, corresponds to aa 280 ... 545, 33% identity) PubMed

P39215 (AlphaFold)

Modification

methylated at residues Gln371, Glu630, and Glu637 PubMed

Effectors of protein activity

asparagine (binds to the part of McpB that is exposed to the exterior) PubMed

Kinetic information

K(D) for asparagine: 14 myM PubMed

Paralogous protein(s)

McpA, TlpB, TlpA, McpC

Localization

cell membrane PubMed

localized at the cell poles, but in the presence of high asparagine concentration there is a reversible re-localization to the sides of the cell (lateral clusters) PubMed

Expression and Regulation

Operons

Genes

mcpB

Description

PubMed

Sigma factors

SigD: sigma factor, PubMed, in sigD regulon

Additional information

in minimal medium, McpB is present with 6,200 /- 1,900 molecules per cell PubMed

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Biological materials