accB

168

acetyl-CoA carboxylase (biotin carboxyl carrier subunit)

Locus: BSU_24350

Molecular weight: 17.09 kDa

Isoelectric point: 4.39

Protein length: 159 aa

Gene length: 480 bp

Function: production of malonyl-CoA, the substrate for fatty acid biosynthesis

Product: acetyl-CoA carboxylase (biotin carboxyl carrier subunit)

Essential: yes

E.C.: 6.4.1.2

Synonyms: accB, fabE, yqhW

Genomic Context

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List of homologs in different organisms, belongs to COG0511 (Galperin et al., 2021)

Gene

Coordinates: 2,531,718  2,532,197

Phenotypes of a mutant

• essential PubMed
• enhanced colony wrinkling upon knockdown PubMed

Additional information

• ponA ecsA, ponA sigI, and ponA rasP double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in accA, accB, accC, accD or downregulation of the FapR regulon due to specific mutations in fapR) PubMed

The protein

Domains

• Biotinyl-binding domain (aa 81-157) (according to UniProt)

Cofactors

• biotin PubMed

Structure

• 1BDO (PDB) (the protein from E. coli) PubMed
• P49786 (AlphaFold)

Additional information

• AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by SPINE.

Expression and Regulation

Operons

• Genes: accB-accC-rfaA

  Description: PubMed

Biological materials

Expression vectors

• GP1477 (chromosomal accB-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
• pGP1027: IPTG inducible expression, purification in E. coli without any tag, in pGP574, available in Jörg Stülke's lab
• pGP2649: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
• pGP2650: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
• pGP2652: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1484 (mls, based on pGP1087), available in Jörg Stülke's lab

GFP fusion

• GP1493 (mls, based on pGP1080), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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