accB

accB
168

acetyl-CoA carboxylase (biotin carboxyl carrier subunit)

Locus
BSU_24350
Molecular weight
17.09 kDa
Isoelectric point
4.39
Protein length
Gene length
Function
production of malonyl-CoA, the substrate for fatty acid biosynthesis
Product
acetyl-CoA carboxylase (biotin carboxyl carrier subunit)
Essential
yes
E.C.
6.4.1.2
Synonyms
accB, fabE, yqhW

Genomic Context

Categories containing this gene/protein

List of homologs in different organisms, belongs to COG0511 (Galperin et al., 2021)

This gene is a member of the following regulons

Gene
Coordinates
2,531,718  2,532,197
Phenotypes of a mutant
essential PubMed
enhanced colony wrinkling upon knockdown PubMed
Additional information
ponA ecsA, ponA sigI, and ponA rasP double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in accA, accB, accC, accD or downregulation of the FapR regulon due to specific mutations in fapR) PubMed
The protein
Biotinyl-binding domain (aa 81-157) (according to UniProt)
biotin PubMed
Structure
1BDO (PDB) (the protein from E. coli) PubMed
Additional information
AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by SPINE.
Expression and Regulation
Operons
Description
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accBrfaA

2025-04-05 17:23:20

ghost

164

86e257ca959326e8b837637c9b51bc6f18c4c8c4

C4E0BC3C47DE59CA027C5A57E3491771B42AECD9

Biological materials
Expression vectors
GP1477 (chromosomal accB-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
pGP1027: IPTG inducible expression, purification in E. coli without any tag, in pGP574, available in Jörg Stülke's lab
pGP2649: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
pGP2650: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
pGP2652: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab
Two-hybrid system
B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
FLAG-tag construct
GP1484 (mls, based on pGP1087), available in Jörg Stülke's lab
GFP fusion
GP1493 (mls, based on pGP1080), available in Jörg Stülke's lab
References
Reviews
Cronan JEThe Classical, Yet Controversial, First Enzyme of Lipid Synthesis: Escherichia coli Acetyl-CoA Carboxylase.Microbiology and molecular biology reviews : MMBR. 2021 Aug 18; 85(3):e0003221. PMID: 34132100
Tong L Acetyl-coenzyme A carboxylase: crucial metabolic enzyme and attractive target for drug discovery. Cellular and molecular life sciences : CMLS. 2005 Aug; 62(16):1784-803. . PMID:15968460
White SW, Zheng J, Zhang YM, Rock The structural biology of type II fatty acid biosynthesis. Annual review of biochemistry. 2005; 74:791-831. . PMID:15952903
Cronan JE, Waldrop GL Multi-subunit acetyl-CoA carboxylases. Progress in lipid research. 2002 Sep; 41(5):407-35. . PMID:12121720
Original Publications
Willdigg JR, Patel Y, Helmann JDA Decrease in Fatty Acid Synthesis Rescues Cells with Limited Peptidoglycan Synthesis Capacity.mBio. 2023 Apr 25; 14(2):e0047523. PMID: 37017514
Arjes HA, Gui H, Porter R, Atolia E, Peters JM, Gross C, Kearns DB, Huang KCFatty Acid Synthesis Knockdown Promotes Biofilm Wrinkling and Inhibits Sporulation in Bacillus subtilis.mBio. 2022 Sep 7; :e0138822. PMID: 36069446
Pisithkul T, Schroeder JW, Trujillo EA, Yeesin P, Stevenson DM, Chaiamarit T, Coon JJ, Wang JD, Amador-Noguez D Metabolic Remodeling during Biofilm Development of Bacillus subtilis. mBio. 2019 May 21; 10(3). pii:e00623-19. doi:10.1128/mBio.00623-19. PMID:31113899
Tödter D, Gunka K, Stülke J The Highly Conserved Asp23 Family Protein YqhY Plays a Role in Lipid Biosynthesis in Bacillus subtilis. Frontiers in microbiology. 2017; 8:883. doi:10.3389/fmicb.2017.00883. PMID:28579978
Henke SK, Cronan JE Successful conversion of the Bacillus subtilis BirA Group II biotin protein ligase into a Group I ligase. PloS one. 2014; 9(5):e96757. doi:10.1371/journal.pone.0096757. PMID:24816803
Athappilly FK, Hendrickson WA Structure of the biotinyl domain of acetyl-coenzyme A carboxylase determined by MAD phasing. Structure (London, England : 1993). 1995 Dec 15; 3(12):1407-19. . PMID:8747466
Marini P, Li SJ, Gardiol D, Cronan JE, de Mendoza D The genes encoding the biotin carboxyl carrier protein and biotin carboxylase subunits of Bacillus subtilis acetyl coenzyme A carboxylase, the first enzyme of fatty acid synthesis. Journal of bacteriology. 1995 Dec; 177(23):7003-6. . PMID:7592499

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Time of last update: 2025-04-09 11:13:42

Author of last update: Jstuelk