accB

168

acetyl-CoA carboxylase (biotin carboxyl carrier subunit)

Locus: BSU_24350

Molecular weight: 17.09 kDa

Isoelectric point: 4.39

Protein length: 159 aa

Gene length: 480 bp

Function: production of malonyl-CoA, the substrate for fatty acid biosynthesis

Product: acetyl-CoA carboxylase (biotin carboxyl carrier subunit)

Essential: yes

E.C.: 6.4.1.2

Synonyms: accB, fabE, yqhW

Genomic Context

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Categories containing this gene/protein

Metabolism, Lipid metabolism, Biosynthesis of lipids, Biosynthesis of fatty acids

Groups of genes, Essential genes

List of homologs in different organisms, belongs to COG0511 (Galperin et al., 2021)

Gene

Coordinates: 2,531,718  2,532,197

Phenotypes of a mutant

essential PubMed
enhanced colony wrinkling upon knockdown PubMed

Additional information

ponA ecsA, ponA sigI, and ponA rasP double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in accA, accB, accC, accD or downregulation of the FapR regulon due to specific mutations in fapR) PubMed

The protein

Domains

Biotinyl-binding domain (aa 81-157) (according to UniProt)

Cofactors

biotin PubMed

Structure

1BDO (PDB) (the protein from E. coli) PubMed
P49786 (AlphaFold)

Additional information

AccB binds to StrepTactin, and may be co-purified when purifying Strep-tagged proteins by SPINE.

Expression and Regulation

Operons

Genes: accB-accC-rfaA

Description: PubMed

Biological materials

Expression vectors

GP1477 (chromosomal accB-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
pGP1027: IPTG inducible expression, purification in E. coli without any tag, in pGP574, available in Jörg Stülke's lab
pGP2649: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
pGP2650: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
pGP2652: IPTG inducible expression, purification in E. coli with C-terminal Strep-tag, in pGP574, available in Jörg Stülke's lab

Two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

GP1484 (mls, based on pGP1087), available in Jörg Stülke's lab

GFP fusion

GP1493 (mls, based on pGP1080), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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