accC

168

acetyl-CoA carboxylase (biotin carboxylase subunit)

Locus: BSU_24340

Molecular weight: 49.41 kDa

Isoelectric point: 4.72

Protein length: 450 aa

Gene length: 1353 bp

Function: production of malonyl-CoA, the substrate for fatty acid biosynthesis

Product: acetyl-CoA carboxylase (biotin carboxylase subunit)

Essential: yes

E.C.: 6.3.4.14

Synonyms: accC, yqhX

Genomic Context

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List of homologs in different organisms, belongs to COG0439 (Galperin et al., 2021)

Gene

Coordinates: 2,530,354  2,531,706

Phenotypes of a mutant

• essential PubMed
• specific point mutations affecting AccC facilitate the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate PubMed
• enhanced colony wrinkling upon knockdown PubMed

Additional information

• ponA ecsA, ponA sigI, and ponA rasP double mutants have a growth defect that can be suppressed by reduced fatty acid synthesis (mutations in accA, accB, accC, accD or downregulation of the FapR regulon due to specific mutations in fapR) PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + hydrogencarbonate + N⁶-biotinyl-L-lysyl-[protein] --> ADP + H⁺ + N⁶-carboxybiotinyl-L-lysyl-[protein] + phosphate (according to UniProt)
• acetyl-CoA + ATP + hydrogencarbonate --> ADP + H⁺ + malonyl-CoA + phosphate (according to UniProt)

Domains

• ATP-grasp domain (aa 120-318) (according to UniProt)
• Biotin carboxylation domain (aa 1-447) (according to UniProt)

Structure

• 1BNC (PDB) (the protein from E. coli) PubMed
• P49787 (AlphaFold)

Paralogous protein(s)

• YngH

Expression and Regulation

Operons

• Genes: accB-accC-rfaA

  Description: PubMed

Biological materials

Expression vectors

• GP1478 (chromosomal accC-Strep fusion, aphA3), purification from B. subtilis, for SPINE, available in Jörg Stülke's lab
• pGP1025, N-terminal Strep-tag, based on pGP172, purification from E. coli, available in Jörg Stülke's lab
• pGP3646 (N-terminal 6xHis-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1483 (spc, based on pGP1331), available in Jörg Stülke's lab

GFP fusion

• GP1491 (spc, based on pBP43), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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