fra

168

frataxin-like protein, required for the biosynthesis of iron-sulfur clusters, intracellular carrier to provide iron for the ferrochelatase HemH

Locus: BSU_05750

Molecular weight: 14.28 kDa

Isoelectric point: 5.38

Protein length: 123 aa

Gene length: 372 bp

Function: intracellular iron channeling, biosynthesis of iron-sulfur clusters

Product: frataxin-like protein

Essential: no

Synonyms: fra, ydhG

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5646 (Galperin et al., 2021)

Gene

Coordinates: 621,847  622,218

Phenotypes of a mutant

• severe growth phenotype associated with a broadly disturbed iron homeostasis PubMed

The protein

Catalyzed reaction/ biological activity

• transfers iron onto the B. subtilis SUF system (SufU-SufS) for iron-sulfur cluster biosynthesis PubMed

Cofactors

• Fe(2+) is bound with high affinity PubMed

Structure

• 2OC6 (PDB)
• Q797E6 (AlphaFold)

Expression and Regulation

Operons

• Genes: fra

  Description: PubMed

Biological materials

Mutant

• GP4705 Δfra::aphA3, available in Jörg Stülke's lab
• MGNA-C182 (ydhG::erm), available at the NBRP B. subtilis, Japan
• BKE05750 (fra::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTTCCCTCCTAAAA,  downstream forward: _UP4_TGAAGAAAAAGGCATAACAT
• BKK05750 (fra::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTGTTCCCTCCTAAAA,  downstream forward: _UP4_TGAAGAAAAAGGCATAACAT

References

Reviews

Loading

Original Publications

Loading