yaaR

168

unknown

Locus: BSU_00300

Molecular weight: 16.58 kDa

Isoelectric point: 9.72

Protein length: 146 aa

Gene length: 441 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yaaR

Genomic Context

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Categories containing this gene/protein

Lifestyles, Exponential and early post-exponential lifestyles, Swarming

Groups of genes, Strongly expressed understudied proteins, Other highly expressed unknown proteins

Groups of genes, Proteins of unknown function

List of homologs in different organisms, belongs to COG1728 (Galperin et al., 2021)

Gene

Coordinates: 40,213  40,653

Phenotypes of a mutant

defective in swarming motility (reduced rate of colony expansion) PubMed

The protein

Structure

2QUP (PDB) (from Bacillus halodurans, 40% identity)
P37539 (AlphaFold)

Expression and Regulation

Operons

Genes: xpaC-yaaN-yaaO-tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

Description: PubMed

Genes: darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

Description: PubMed

Regulation

expressed during growth and the transition phase, expression is erduced in stationary phase PubMed

Genes: tmk-darA-yaaR-holB-ricT-yabA-trmN6-yazA-yabC

Description: PubMed

Biological materials

Mutant

MGNA-B898 (yaaR::erm), available at the NBRP B. subtilis, Japan
GP1713 (''darA-yaaR::cat''), available in Jörg Stülke's lab
BKE00300 (''yaaR::erm'', available in the BGSC and in Jörg Stülke's lab) PubMed
BKE00300 (yaaR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTTTCAGATCCTTAAA,  downstream forward: _UP4_CTTTACACATAGAGAGTGAT
BKK00300 (yaaR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACAGTTTCAGATCCTTAAA,  downstream forward: _UP4_CTTTACACATAGAGAGTGAT

Expression vectors

pGP2673: expression of yaaR-Strep by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

Two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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