cheR

168

MCPs methyltransferase

Locus: BSU_22720

Molecular weight: 29.80 kDa

Isoelectric point: 9.25

Protein length: 256 aa

Gene length: 771 bp

Function: motility, chemotaxis

Product: MCPs methyltransferase

Essential: no

E.C.: 2.1.1.80

Synonyms: cheR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1352 (Galperin et al., 2021)

Gene

Coordinates: 2,380,347  2,381,117

The protein

Catalyzed reaction/ biological activity

• methylation of specific glutamate residues in the cytoplasmic domain of methyl-accepting chemotactic protein receptors (MCPRs)
• L-glutamyl-[protein] + S-adenosyl-L-methionine --> [protein]-L-glutamate 5-O-methyl ester + S-adenosyl-L-homocysteine (according to UniProt)

Domains

• CheR-type methyltransferase domain (aa 1-256) (according to UniProt)

Structure

• 5FTW (PDB) (complex with S-adenosyl-L-homocysteine)
• P31105 (AlphaFold)

Expression and Regulation

Operons

• Genes: cheR-aroF-aroB-aroH

  Description: PubMed

Biological materials

Mutant

• 1A830 ( cheR::cat), PubMed, available at BGSC
• BKE22720 (cheR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTACTCTCCCAGTCT,  downstream forward: _UP4_TAGACTGACTTTTTGCTGAA
• BKK22720 (cheR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTCTACTCTCCCAGTCT,  downstream forward: _UP4_TAGACTGACTTTTTGCTGAA

References

Reviews

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Original Publications

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