yrrK

168

processing of the 5 end of pre-16S rRNA (based on similarity to E. coli YqgF)

Locus: BSU_27390

Molecular weight: 15.07 kDa

Isoelectric point: 5.32

Protein length: 138 aa

Gene length: 417 bp

Function: 16S rRNA maturation

Product: putative RNase

Essential: no

Synonyms: yrrK

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0816 (Galperin et al., 2021)

Gene

Coordinates: 2,797,399  2,797,815

The protein

Protein family

• YqgF nuclease family (single member, according to UniProt)

Domains

• YqgF/RNase H-like domain (aa 1 ... 103) (according to the Interpro database)

Structure

• 1VHX (PDB) PubMed
• O34634 (AlphaFold)

Expression and Regulation

Operons

• Genes: reoM-yrrK-yrzB

  Description: PubMed

• Genes: alaS-reoM-yrrK-yrzB

  Description: PubMed

  Regulation

  • induced by alanine limitation ([regulon|T-box|]) PubMed

  Regulatory mechanism

  • T-box: RNA switch, [pubmed|], in T-box

Biological materials

Mutant

• MGNA-B514 (yrrK::erm), available at the NBRP B. subtilis, Japan
• BKE27390 (yrrK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGTCCTAATATTCTCATTC,  downstream forward: _UP4_AATTAAAGCCAGAGGTGAAG
• BKK27390 (yrrK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GAGTCCTAATATTCTCATTC,  downstream forward: _UP4_AATTAAAGCCAGAGGTGAAG

References

Loading