Difference between revisions of "YsxC"

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* '''Description:''' assembly of the 50S subunit of the ribosome <br/><br/>
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* '''Description:''' assembly of the 50S subunit of the [[ribosome]] <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || GTPase  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || GTPase  
 
|-
 
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|style="background:#ABCDEF;" align="center"|'''Function''' || ribosome assembly
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|style="background:#ABCDEF;" align="center"|'''Function''' || [[ribosome]] assembly
 
|-
 
|-
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28190 ysxC]
 
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU28190 ysxC]
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= [[Categories]] containing this gene/protein =
 
= [[Categories]] containing this gene/protein =
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=== Additional information===
 
=== Additional information===
 
 
  
  
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==Original publications==
 
==Original publications==
 
'''Additional publications:''' {{PubMed|21636901}}  
 
'''Additional publications:''' {{PubMed|21636901}}  
<pubmed>17981968,11073929 ,12427945, 16997968 7961402 9852015 14684918 15136032 </pubmed>
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<pubmed>17981968,11073929 ,12427945, 16997968 7961402 9852015 14684918 15136032 21636901 25771857</pubmed>
  
 
[[Category:Protein-coding genes]]
 
[[Category:Protein-coding genes]]

Revision as of 07:26, 17 March 2015

  • Description: assembly of the 50S subunit of the ribosome

Gene name ysxC
Synonyms orfX
Essential yes PubMed
Product GTPase
Function ribosome assembly
Gene expression levels in SubtiExpress: ysxC
Interactions involving this protein in SubtInteract: YsxC
Metabolic function and regulation of this protein in SubtiPathways:
ysxC
MW, pI 21 kDa, 9.708
Gene length, protein length 585 bp, 195 aa
Immediate neighbours ysxD, lonA
Sequences Protein DNA DNA_with_flanks
Genetic context
YsxC context.gif
Expression at a glance   PubMed
YsxC expression.png















Categories containing this gene/protein

translation, essential genes, membrane proteins, GTP-binding proteins

This gene is a member of the following regulons

CtsR regulon

The gene

Basic information

  • Locus tag: BSU28190

Phenotypes of a mutant

essential PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity: Binds and hydrolyzes GTP and readily exchanges GDP for GTP
  • Protein family: engB family (according to Swiss-Prot) Era/Obg family

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure: 1SVI (complex with GDP), 1SUL
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 114 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 113 PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • for expression/ purification from B. subtilis with N-terminal Strep-tag, for SPINE, in pGP380: pGP834, available in Stülke lab
    • for expression/ purification from E. coli with N-terminal His-tag, in pWH844: pGP842, available in Stülke lab
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Naotake Ogasawara, Nara, Japan

Your additional remarks

References

Reviews

Natalie Verstraeten, Maarten Fauvart, Wim Versées, Jan Michiels
The universally conserved prokaryotic GTPases.
Microbiol Mol Biol Rev: 2011, 75(3);507-42, second and third pages of table of contents
[PubMed:21885683] [WorldCat.org] [DOI] (I p)

Robert A Britton
Role of GTPases in bacterial ribosome assembly.
Annu Rev Microbiol: 2009, 63;155-76
[PubMed:19575570] [WorldCat.org] [DOI] (I p)


Original publications

Additional publications: PubMed