comP

168

two-component sensor kinase, detects extracellular ComX

Locus: BSU_31690

Molecular weight: 89.13 kDa

Isoelectric point: 8

Protein length: 769 aa

Gene length: 2310 bp

Function: regulation of genetic competence and quorum sensing

Product: two-component sensor kinase

Essential: no

Synonyms: comP

Genomic Context

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List of homologs in different organisms, belongs to COG4585 (Galperin et al., 2021)

Gene

Coordinates: 3,253,529  3,255,838

Phenotypes of a mutant

• the mutation suppresses the mucoid phenotype of ''motA or motB'' mutants due to loss of DegU phosphorylation and concomitant reduced expression of the ''capB-capC-capA-capE'' operon PubMed

The protein

Catalyzed reaction/ biological activity

• autophosphorylation
• phosphorylation of ComA when bound to ComX
• ATP + protein L-histidine --> ADP + protein N-phospho-L-histidine (according to UniProt)

Domains

• eight transmembrane segments
• Histidine kinase domain (aa 571-769) (according to UniProt)

Structure

• Q99027 (AlphaFold)

Modification

• autophosphorylation on a His residue

Effectors of protein activity

• ComX (extracellular)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: comQ-comX-comP-comA-yuxO

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE31690 (comP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATATTAATCCACCTATTA,  downstream forward: _UP4_TAATGGATTTATAACGGAAA
• BKK31690 (comP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATATTAATCCACCTATTA,  downstream forward: _UP4_TAATGGATTTATAACGGAAA

References

Reviews

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Original Publications

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