comA

168

two-component system response regulator, controls gene expression in response to cell density (concentration of ComX)

Locus: BSU_31680

Molecular weight: 23.98 kDa

Isoelectric point: 4.76

Protein length: 214 aa

Gene length: 645 bp

Function: regulation of genetic competence and quorum sensing

Product: two-component system response regulator

Essential: no

E.C.: 4.4.1.19

Synonyms: comA, srfB, comAA

Genomic Context

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List of homologs in different organisms, belongs to COG2197 (Galperin et al., 2021)

Gene

Coordinates: 3,252,804  3,253,448

The protein

Domains

• Response regulatory domain (aa 3-121) (according to UniProt)
• HTH luxR-type domain (aa 147-212) (according to UniProt)

Structure

• 1U83 (PDB)
• 3ULQ (PDB) (structure of the complex between the DNA-binding C-terminal domain of ComA with RapF) PubMed
• P14204 (AlphaFold)

Modification

• phosphorylated by ComP on an Asp residue

Effectors of protein activity

• phosphorylation activates DNA binding
• DNA-binding activity of ComA is inhibited by RapC PubMed, RapD PubMed, RapF PubMed, RapH PubMed and RapK PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: comQ-comX-comP-comA-yuxO

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE31680 (comA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCCTCCCTTTTAC,  downstream forward: _UP4_CTTTAAATGTTGGGGGGTGT
• BKK31680 (comA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTTTTCCTCCCTTTTAC,  downstream forward: _UP4_CTTTAAATGTTGGGGGGTGT

References

Reviews

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Original Publications

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