Difference between revisions of "YqhY"

From SubtiWiki
Jump to: navigation, search
(Biological materials)
(Biological materials)
Line 132: Line 132:
 
** pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab  
 
** pGP1325 (N-terminal His-tag, purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab  
 
** pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [[Jörg Stülke]]'s lab
 
** pGP1496 (His-tag, purification from ''E. coli'', in pET28a+), available in [[Jörg Stülke]]'s lab
 +
** pGP1498 (N-terminal His-tag, TEV-site, purification from ''E. coli'', in [[pWH844]]), available in [[Jörg Stülke]]'s lab
 
          
 
          
 
* '''lacZ fusion:'''
 
* '''lacZ fusion:'''

Revision as of 12:57, 6 March 2015

  • Description: unknown

Gene name yqhY
Synonyms
Essential no PubMed
Product unknown
Function unknown
Gene expression levels in SubtiExpress: yqhY
Metabolic function and regulation of this protein in SubtiPathways:
yqhY
MW, pI 14 kDa, 4.414
Gene length, protein length 405 bp, 135 aa
Immediate neighbours nusB, accC
Sequences Protein DNA DNA_with_flanks
Genetic context
YqhY context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
YqhY expression.png















Categories containing this gene/protein

proteins of unknown function

This gene is a member of the following regulons

The gene

Basic information

  • Locus tag: BSU24330

Phenotypes of a mutant

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: asp23 family (according to Swiss-Prot)
  • Paralogous protein(s): YloU

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Cofactor(s):
  • Effectors of protein activity:

Database entries

  • Structure:
  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 127 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 893 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, exponential phase): 4905 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, early stationary phase after glucose exhaustion): 3644 PubMed
    • number of protein molecules per cell (minimal medium with glucose and ammonium, late stationary phase after glucose exhaustion): 4928 PubMed

Biological materials

  • Mutant:
  • Expression vector:
    • pGP1322 (N-terminal Strep-tag, purification from E. coli, in pGP172), available in Jörg Stülke's lab
    • pGP1325 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab
    • pGP1496 (His-tag, purification from E. coli, in pET28a+), available in Jörg Stülke's lab
    • pGP1498 (N-terminal His-tag, TEV-site, purification from E. coli, in pWH844), available in Jörg Stülke's lab
  • lacZ fusion:
  • two-hybrid system: B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab
  • Antibody:

Labs working on this gene/protein

Your additional remarks

References