proI

168

pyrroline-5-carboxylate reductase

Locus: BSU_23800

Molecular weight: 30.24 kDa

Isoelectric point: 6.7

Protein length: 278 aa

Gene length: 837 bp

Function: biosynthesis of proline

Product: pyrroline-5-carboxylate reductase

Essential: no

E.C.: 1.5.1.2

Synonyms: proI, yqjO

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0345 (Galperin et al., 2021)

Gene

Coordinates: 2,473,151  2,473,987

The protein

Catalyzed reaction/ biological activity

• 1-pyrroline-5-carboxylate + NADPH + H⁺ --> L-proline + NADP⁺, activity is biologically not relevant PubMed

Protein family

• pyrroline-5-carboxylate reductase family PubMed

Cofactors

• NADPH PubMed

Structure

• 5BSE (PDB) (from Medicago truncatula, 33% identity) PubMed
• P54552 (AlphaFold)

Paralogous protein(s)

• ProH

Expression and Regulation

Operons

• Genes: proI

  Description: PubMed

  Regulation

  • induced by proline limitation (T-box) PubMed

  Regulatory mechanism

  • T-box: RNA switch, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C395 (yqjO::erm), available at the NBRP B. subtilis, Japan
• BKE23800 (proI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATATCCTCCTTCTAT,  downstream forward: _UP4_TAGATGTAAGGACAAAAACA
• BKK23800 (proI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGATATCCTCCTTCTAT,  downstream forward: _UP4_TAGATGTAAGGACAAAAACA
• GP3748 (proI::aphA3 trpC2) available in the Stülke lab

Expression vectors

• pGP3799: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
• pGP3861: expression of Strep-proI by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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