proG

168

1-pyrroline-5-carboxylate dehydrogenase

Locus: BSU_12910

Molecular weight: 30.05 kDa

Isoelectric point: 4.91

Protein length: 272 aa

Gene length: 819 bp

Function: biosynthesis of proline

Product: 1-pyrroline-5-carboxylate dehydrogenase

Essential: no

E.C.: 1.5.1.2

Synonyms: proG, ykeA, yzcA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0345 (Galperin et al., 2021)

Gene

Coordinates: 1,359,454  1,360,272

The protein

Catalyzed reaction/ biological activity

• 1-pyrroline-5-carboxylate + NADPH + H⁺ --> L-proline + NADP⁺ PubMed
• L-proline + NADP⁺ --> 1-pyrroline-5-carboxylate + 2 H⁺ + NADPH (according to UniProt)
• L-proline + NAD⁺ --> 1-pyrroline-5-carboxylate + 2 H⁺ + NADH (according to UniProt)

Protein family

• pyrroline-5-carboxylate reductase family PubMed

Cofactors

• NADPH PubMed

Structure

• Q00777 (AlphaFold)

Effectors of protein activity

• feedback-inhibited by proline PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: proG

  Description: PubMed

Biological materials

Mutant

• MGNA-B308 (ykeA::erm), available at the NBRP B. subtilis, Japan
• BKE12910 (proG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT,  downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
• BKK12910 (proG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT,  downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
• GP3728 (proG::tet trpC2) available in the Stülke lab
• GP3741 (proG::aphA3 trpC2) available in the Stülke lab

Expression vectors

• pGP3791: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab
• pGP3863: expression of Strep-proG by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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