proH

168

pyrroline-5-carboxylate reductase

Locus: BSU_18480

Molecular weight: 31.88 kDa

Isoelectric point: 7.75

Protein length: 297 aa

Gene length: 894 bp

Function: osmoadaptive de novo production of proline

Product: pyrroline-5-carboxylate reductase

Essential: no

E.C.: 1.5.1.2

Synonyms: proH, yoxE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0345 (Galperin et al., 2021)

Gene

Coordinates: 2,016,845  2,017,738

The protein

Catalyzed reaction/ biological activity

• 1-pyrroline-5-carboxylate + NAD(P)H + H⁺ --> L-proline + NAD(P)⁺ PubMed

Protein family

• pyrroline-5-carboxylate reductase family PubMed

Cofactors

• NADPH PubMed

Structure

• 5BSE (PDB) (from Medicago truncatula, 36% identity) PubMed
• P0CI77 (AlphaFold)

Effectors of protein activity

• feedback-inhibited by proline PubMed

Paralogous protein(s)

• ProI

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: proH-proJ

  Description: PubMed

  Regulation

  • expressed under conditions of osmotic stress PubMed
  • induced upon antibiotic stress PubMed

  Expression

  • expression is heterogeneously activated PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• HB24914 (proH::erm), available in John Helmann's and Jörg Stülke's labs
• MGNA-B083 (proH::erm), available at the NBRP B. subtilis, Japan
• BKE18480 (proH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCACACGTTTAAAAT,  downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
• BKK18480 (proH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTCACACGTTTAAAAT,  downstream forward: _UP4_GCGCCGCTATCAGGAGTGAT
• GP3709 (proH::aphA3 trpC2) available in the Stülke lab

Expression vectors

• pGP3800: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
• pGP3862: expression of Strep-proH by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab

References

Reviews

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Original Publications

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