Difference between revisions of "TyrZ"
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| − | * '''Description:''' tyrosyl-tRNA synthetase | + | * '''Description:''' alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins <br/><br/> |
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= [[Categories]] containing this gene/protein = | = [[Categories]] containing this gene/protein = | ||
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= This gene is a member of the following [[regulons]] = | = This gene is a member of the following [[regulons]] = | ||
| + | {{SubtiWiki regulon|[[DtrR regulon]]}}, | ||
{{SubtiWiki regulon|[[T-box]]}} | {{SubtiWiki regulon|[[T-box]]}} | ||
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=== Additional information=== | === Additional information=== | ||
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=Expression and regulation= | =Expression and regulation= | ||
| − | * '''Operon:''' | + | * '''Operon:''' ''[[dtrR]]-[[tyrZ]]'' {{PubMed|25733610}} |
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tyrZ_3947158_3948399_1 tyrZ] {{PubMed|22383849}} | * '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=tyrZ_3947158_3948399_1 tyrZ] {{PubMed|22383849}} | ||
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* '''Regulatory mechanism:''' | * '''Regulatory mechanism:''' | ||
** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532,1735721}} | ** [[T-box]]: [[RNA switch]], transcriptional antitermination {{PubMed|19258532,1735721}} | ||
| + | ** [[DtrR]]: transcriptional repression {{PubMed|25733610}} | ||
* '''Additional information:''' | * '''Additional information:''' | ||
Revision as of 13:02, 4 March 2015
- Description: alternative tyrosyl-tRNA synthetase, required to prevent misincorporation of D-Tyr into proteins
| Gene name | tyrZ |
| Synonyms | ipa-9r, tyrS1, tyrT |
| Essential | no |
| Product | tyrosyl-tRNA synthetase (minor) |
| Function | translation |
| Gene expression levels in SubtiExpress: tyrZ | |
| Metabolic function and regulation of this protein in SubtiPathways: tyrZ | |
| MW, pI | 46 kDa, 6.661 |
| Gene length, protein length | 1239 bp, 413 aa |
| Immediate neighbours | ywaE, ywaD |
| Sequences | Protein DNA DNA_with_flanks |
Genetic context 
This image was kindly provided by SubtiList
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Expression at a glance PubMed
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Contents
Categories containing this gene/protein
This gene is a member of the following regulons
The gene
Basic information
- Locus tag: BSU38460
Phenotypes of a mutant
Database entries
- BsubCyc: BSU38460
- DBTBS entry: [1]
- SubtiList entry: [2]
Additional information
The protein
Basic information/ Evolution
- Catalyzed reaction/ biological activity: ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot) ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr) (according to Swiss-Prot)
- Protein family: TyrS type 2 subfamily (according to Swiss-Prot), class-I aminoacyl-tRNA synthetase family (according to Swiss-Prot)
- Paralogous protein(s):
Extended information on the protein
- Kinetic information:
- Domains:
- Modification: phosphorylated on Thr-311 PubMed
- Cofactor(s):
- Effectors of protein activity:
- Localization:
- cytoplasm (according to Swiss-Prot)
Database entries
- BsubCyc: BSU38460
- Structure:
- UniProt: P25151
- KEGG entry: [3]
- E.C. number: 6.1.1.1
Additional information
Expression and regulation
- Regulatory mechanism:
- T-box: RNA switch, transcriptional antitermination PubMed
- DtrR: transcriptional repression PubMed
- Additional information:
Biological materials
- Mutant: QB53532 (aphA3), available in the Stülke lab
- Expression vector:
- lacZ fusion:
- GFP fusion:
- two-hybrid system:
- Antibody:
Labs working on this gene/protein
Your additional remarks
References
