ykwC

168

beta-hydroxyacid dehydrogenase

Locus: BSU_13960

Molecular weight: 30.56 kDa

Isoelectric point: 5.11

Protein length: 288 aa

Gene length: 867 bp

Function: unknown

Product: beta-hydroxyacid dehydrogenase

Essential: no

E.C.: 1.1.-.-

Synonyms: ykwC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2084 (Galperin et al., 2021)

Gene

Coordinates: 1,465,733 1,466,599

The protein

Protein family

• HIBADH-related family (with YfjR, according to UniProt)

Structure

• 3WS7 (PDB) (from Pyrobaculum calidifontis , 42% identity)
• O34948 (AlphaFold)

Modification

• phosphorylated on Ser-281 by PrkC, dephosphorylated by PrpC, phosphorylation abolishes the oxidoreductase activity of YkwC in vitro and in vivo PubMed

Paralogous protein(s)

• YfjR, (32%)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: ykwC

  Description: PubMed

Biological materials

Mutant

• MGNA-B333 (ykwC::erm), available at the NBRP B. subtilis, Japan
• BKE13960 (ykwC::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGGTCATTCCTCCCAGAT, downstream forward: _UP4_TAAAAAAAGATTGCCTGTAC
• BKK13960 (ykwC::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAGGTCATTCCTCCCAGAT, downstream forward: _UP4_TAAAAAAAGATTGCCTGTAC

References

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