cdoA

168

cysteine dioxygenase

Locus: BSU_31140

Molecular weight: 17.98 kDa

Isoelectric point: 5.05

Protein length: 161 aa

Gene length: 486 bp

Function: unknown

Product: cysteine dioxygenase

Essential: no

Synonyms: yubC

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5553 (Galperin et al., 2021)

Gene

Coordinates: 3,193,863  3,194,348

The protein

Catalyzed reaction/ biological activity

• L-cysteine + O₂ --> cysteine sulfinic acid + H⁺ PubMed

Protein family

• cysteine dioxygenase family (single membran, according to UniProt)

Structure

• 3EQE (PDB)
• 4QM8 PubMed, 4QM9 (PDB) (from Bacillus Subtilis 100% identity)
• O32085 (AlphaFold)

Expression and Regulation

Operons

• Genes: cdoA

  Description: PubMed

Biological materials

Mutant

• MGNA-A225 (yubC::erm), available at the NBRP B. subtilis, Japan
• BKE31140 (cdoA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCGTTTTTGCCTCCCTT,  downstream forward: _UP4_TGATTGACAGATAAACTGCT
• BKK31140 (cdoA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCGTTTTTGCCTCCCTT,  downstream forward: _UP4_TGATTGACAGATAAACTGCT

References

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