melE

168

subunit of ABC transporter for melibiose and raffinose (binding lipoprotein)

Locus: BSU_30270

Molecular weight: 48.07 kDa

Isoelectric point: 5.85

Protein length: 426 aa

Gene length: 1281 bp

Function: uptake of melibiose and raffinose

Product: ABC transporter (binding protein)

Essential: no

Synonyms: melE, msmE

Genomic Context

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List of homologs in different organisms, belongs to COG1653 (Galperin et al., 2021)

Gene

Coordinates: 3,097,850 → 3,099,130

The protein

Catalyzed reaction/ biological activity

• binding of melibiose and raffinose PubMed

Protein family

• Bacterial solute-binding protein 1 family (according to UniProt)

Structure

• 4R6H (PDB)
• O34335 (AlphaFold)

Localization

• extracellular (signal peptide), associated to the cell membrane (via MelC-MelD) PubMed

Expression and Regulation

Operons

• Genes: melE-melD-melC-melA

  Description: PubMed

  Regulation

  • repressed by glucose (CcpA) PubMed

• Genes: melR-melE-melD-melC-melA

  Description: PubMed

  Regulation

  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon
  • MelR: repression, PubMed, in melR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A801 (msmE::erm), available at the NBRP B. subtilis, Japan
• BKE30270 (ΔmelE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT,  downstream forward: _UP4_TCCGAAACACAGGGAGCTGA
• BKK30270 (ΔmelE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTTTTCCTCCTTATCAT,  downstream forward: _UP4_TCCGAAACACAGGGAGCTGA

References

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