fliJ

168

flagellar protein required for formation of basal body, part of the ATPase complex, regulator of the FliI ATPase

Locus: BSU_16250

Molecular weight: 17.77 kDa

Isoelectric point: 8.41

Protein length: 147 aa

Gene length: 444 bp

Function: movement and chemotaxis

Product: flagellar protein required for formation of basal body

Essential: no

Synonyms: fliJ, cheF

Genomic Context

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List of homologs in different organisms, belongs to COG2882 (Galperin et al., 2021)

Gene

Coordinates: 1,697,196  1,697,639

Phenotypes of a mutant

• defective in Swarming motility PubMed

The protein

Protein family

• FliJ family (single member, according to UniProt)

Structure

• 3AJW (PDB) (from S. typhimurium, 19% identity, 63% similarity) PubMed
• P20487 (AlphaFold)

Localization

• cytoplasm, as part of the flagellum attached to the membrane PubMed

Expression and Regulation

Operons

• Genes: flgB-flgC-fliE-fliF-fliG-fliH-fliI-fliJ-ylxF-fliK-flgD-flgE-swrD-fliL-fliM-fliY-cheY-fliO-fliP-fliQ-fliR-flhB-flhA-flhF-flhG-cheB-cheA-cheW-cheC-cheD-sigD-swrB

  Description: PubMed

  Regulation

  • expression during spore germination is strongly reduced under conditions of osmotic stress PubMed

  Regulatory mechanism

  • Spo0A: repression, in spo0A regulon
  • SwrA: activation, in swrA regulon
  • CodY: repression, in codY regulon
  • DegU: repression, in degU regulon
  • SinR: transcription elongation, binding of the SinR-SlrR heteromer to sites within fliE and fliI results in inhibition of transcription elongation PubMed, in sinR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• BKE16250 (fliJ::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTATTCCTCATTTCC,  downstream forward: _UP4_CAAGGCCATTAGGAGCGAAT
• BKK16250 (fliJ::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACGTTTATTCCTCATTTCC,  downstream forward: _UP4_CAAGGCCATTAGGAGCGAAT

References

Reviews

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Original Publications

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