phoD

168

phosphodiesterase/alkaline phosphatase, degrades wall teichoic acid during phosphate starvation

Locus: BSU_02620

Molecular weight: 62.66 kDa

Isoelectric point: 8.39

Protein length: 583 aa

Gene length: 1752 bp

Function: aquisition of phosphate upon phosphoate starvation, degradation of wall teichoic acid during phosphate starvation

Product: phosphodiesterase/alkaline phosphatase

Essential: no

E.C.: 3.1.3.1

Synonyms: phoD, ycbS

Genomic Context

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List of homologs in different organisms, belongs to COG3540 (Galperin et al., 2021)

Gene

Coordinates: 284,011  285,762

The protein

Catalyzed reaction/ biological activity

• endohydrolytic activity on wall teichoic acid, acts in concert with GlpQ PubMed
• phosphate monoester + H₂O --> alcohol + phosphate (according to UniProt)

Protein family

• PhoD family (single member, according to UniProt)

Cofactors

• one Fe³⁺ and two Ca²⁺ PubMed

Structure

• 2YEQ (PDB) PubMed
• P42251 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed, secreted by the TatAD-TatCD complex  PubMed

Expression and Regulation

Operons

• Genes: phoD-tatAD-tatCD

  Description: PubMed

  Regulation

  • expressed under conditions of phosphate limitation (PhoP) PubMed

  Regulatory mechanism

  • PhoP: activation, PubMed, in phoP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • expression of the operon depends on functional HemAT and CsbC PubMed

Biological materials

Mutant

• BKE02620 (phoD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC,  downstream forward: _UP4_ATCACGAATTAAGGAGTGGA
• BKK02620 (phoD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCAAGATCCCCTCTCTC,  downstream forward: _UP4_ATCACGAATTAAGGAGTGGA

Labs working on this gene/protein

• Oscar Kuipers, University of Groningen, The Netherlands
• Homepage

References

Reviews

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Original Publications

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