pstS

168

phosphate ABC transporter (binding protein)

Locus: BSU_24990

Molecular weight: 31.53 kDa

Isoelectric point: 4.84

Protein length: 300 aa

Gene length: 903 bp

Function: high-affinity phosphate uptake

Product: phosphate ABC transporter (binding protein)

Essential: no

Synonyms: pstS, yqgG, yzmB

Genomic Context

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List of homologs in different organisms, belongs to COG0226 (Galperin et al., 2021)

Gene

Coordinates: 2,580,715 → 2,581,617

The protein

Protein family

• pstS family (single member, according to UniProt)

Structure

• 4ECF (PDB) (from Lactobacillus brevis, 52% identity)
• P46338 (AlphaFold)

Modification

• phosphorylated on ser/ thr/ tyr PubMed

Localization

• cell membrane (according to UniProt)
• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: pstS-pstC-pstA-pstBA-pstBB

  Description: PubMed

  Regulation

  • expressed under conditions of phosphate limitation (5000-fold induced) (PhoP) PubMed

  Regulatory mechanism

  • PhoP: activation, PhoP-P, PubMed, in phoP regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

  Additional information

  • mRNA processing between pstS and pstC to maintain higher concentrations of PstS relative to other components of the ABC transporter PubMed

Biological materials

Mutant

• MGNA-C420 (yqgG::erm), available at the NBRP B. subtilis, Japan
• BKE24990 (ΔpstS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCTGAATTCCCCCTG,  downstream forward: _UP4_TGATCTTAATAAAAAGAGGA
• BKK24990 (ΔpstS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCCTGAATTCCCCCTG,  downstream forward: _UP4_TGATCTTAATAAAAAGAGGA

Labs working on this gene/protein

• Marion Hulett, University of Illinois at Chicago, USA Homepage

References

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