SasA

• Description: (p)ppGpp synthetase, small alarmone synthetase
  
  

Gene name	sasA
Synonyms	ywaC, ipa-7d
Essential	no
Product	(p)ppGpp synthetase
Function	ppGpp synthesis independent from stringent response
Gene expression levels in SubtiExpress: sasA
MW, pI	24 kDa, 6.217
Gene length, protein length	630 bp, 210 aa
Immediate neighbours	ywaD, menA
Sequences	Protein DNA DNA_with_flanks
Genetic context This image was kindly provided by SubtiList
Expression at a glance   PubMed

Categories containing this gene/protein

metabolism of signalling nucleotides, cell envelope stress proteins (controlled by SigM, V, W, X, Y)

This gene is a member of the following regulons

SigM regulon SigW regulon

The gene

Basic information

• Locus tag: BSU38480

Phenotypes of a mutant

• a relA sasA sasB triple mutant requires branched chain amino acids, methionine and threonine for growth, the requirement can be suppressed by reduced expression of guaB or inactivation of codY PubMed
• a relA sasA sasB triple mutant acquires suppressor mutations in guaA, guaB, gmk or inactivation of codY PubMed

Database entries

• DBTBS entry: [1]

• SubtiList entry: [2]

The protein

Basic information/ Evolution

• Catalyzed reaction/ biological activity:

• Protein family:

• Paralogous protein(s): SasB

Extended information on the protein

• Kinetic information:

• Domains: single ppGpp synthetase domain PubMed

• Modification:

• Cofactors:

• Effectors of protein activity:

• Interactions:

• Localization:

Database entries

• Structure:

• UniProt: P39583

• KEGG entry: [3]

• E.C. number:

Expression and regulation

• Operon: sasA PubMed

• Expression browser: sasA PubMed

• Sigma factor: SigM PubMed SigW PubMed

• Regulation:
  • transcription is highly upregulated in response to lesions in late-acting teichoic acid synthesis genes tagB, tagD and tagF, and also in response to cell-wall-active compounds like bacitracin or vancomycin PubMed
  • expression is reduced in a SigV mutant PubMed

• Regulatory mechanism:

• Additional information:

Biological materials

• Mutant:

• Expression vector:

• lacZ fusion:

• GFP fusion:

• two-hybrid system:

• Antibody: